Supermolecular organization of photosystem II and its associated light-harvesting antenna in Arabidopsis thaliana.

A.E. Yakushevska, P.E. Jensen, W. Keegstra, H. van Roon, H.V. Scheller, E.J. Boekema, J.P. Dekker

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The organization of Arabidopsis thaliana photosystem II (PSII) and its associated light-harvesting antenna (LHCII) was studied in isolated PSII-LHCII supercomplexes and native membrane-bound crystals by transmission electron microscopy and image analysis. Over 4000 single-particle projections of PSII-LHCII supercomplexes were analyzed. In comparison to spinach supercomplexes [Boekema, E.J., van Roon, H., van Breemen, J.F.L. & Dekker, J.P. (1999) Eur. J. Biochem. 266, 444-452] some striking differences were revealed: a much larger number of supercomplexes from Arabidopsis contain copies of M-type LHCII trimers. M-type trimers can also bind in the absence of the more common S-type trimers. No binding of L-type trimers could be detected. Analysis of native membrane-bound PSII crystals revealed a novel type of crystal with a unit cell of 25.6 × 21.4 nm (angle 77°), which is larger than any of the PSII lattices observed before. The data show that the unit cell is built up from C
Original languageEnglish
Pages (from-to)6020-6028
JournalEuropean Journal of Biochemistry
Volume268
DOIs
Publication statusPublished - 2001

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