Abstract
The construction and performance of an automated low-cost Spreeta™-based prototype biosensor system for the detection of endocrine disrupting chemicals (EDCs) is described. The system consists primarily of a Spreeta miniature liquid sensor incorporated into an aluminum flow cell holder, dedicated to support a Biacore chip frame, in combination with a simple pressurized air-driven fluid system. During the optimization, a monoclonal antibody (MAb)-based immunoassay for the estrogenic compound bisphenol A (BPA) was used as a model. After the optimization two thyroxine transport protein inhibition assays for thyroid endocrine disruptors were implemented. The average noise of the system for 1 min of baseline was 1.1 μRIU (refractive index units) and it could be operated in the range of 18-22 °C with a minimum baseline drift (5-10 μRIU/100 min). Optimum signal to noise ratio (S/N R) was obtained using a flow cell height of 100 μm and a flow rate of 180 μl/min. The sensitivity of the Spreeta-based biosensor inhibition assays implemented (50% inhibition concentration (IC
Original language | English |
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Pages (from-to) | 1908-15 |
Journal | Biosensors and Bioelectronics |
Volume | 22 |
Issue number | 9-10 |
DOIs | |
Publication status | Published - 2007 |