The primary photophysics of the Avena sativa phototropin 1 LOV2 domain observed with time-resolved emission spectroscopy

I.H.M. van Stokkum, M. Gauden, S. Crosson, R. van Grondelle, K. Moffat, J.T.M. Kennis

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The phototropins are blue-light receptors that base their light-dependent action on the reversible formation of a covalent bond between a flavin mononucleotide (FMN) cofactor and a conserved cysteine in light, oxygen or voltage (LOV) domains. The primary reactions of the Avena sativa phototropin 1 LOV2 domain were investigated by means of time-resolved and low-temperature fluorescence spectroscopy. Synchroscan streak camera experiments revealed a fluorescence lifetime of 2.2 ns in LOV2. A weak long-lived component with emission intensity from 600 to 650 nm was assigned to phosphorescence from the reactive FMN triplet state. This observation allowed determination of the LOV2 triplet state energy level at physiological temperature at 16600 cm
Original languageEnglish
Pages (from-to)534-541
Number of pages8
JournalPhotochemistry and Photobiology
Volume87
Issue number3
DOIs
Publication statusPublished - 2011

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