Population substructures in the soil invertebrate Orchesella cincta, as revealed by microsatellite and TE-AFLP markers.

A.W.G. van der Wurff, J.A. Isaaks, G. Ernsting, N.M. van Straalen

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Microsatellite and three enzyme-amplified fragment length polymorphism (TE-AFLP) DNA markers were used to describe the population genetic structure in the soil dwelling collembolan Orchesella cincta (L.). Two forests were sampled according to a three-level nested hierarchical design, with fixed distances among samples within a parcel and among parcels within a forest. The largest component of variation was found at the smallest scale, within parcels (77-97%), while the smallest component of variation was found between forests. The two different methods to study population structure indicated a similar allocation of variance. Population genetic substructuring was revealed between samples on a scale of 50 m; the degree of substructuring however, varied between parcels and forests. One forest showed a high degree of structure as revealed by microsatellites, while another showed a low degree of structure. A significant deviation from random-mating (average F
    Original languageEnglish
    Pages (from-to)1349-1359
    JournalMolecular Ecology
    Volume12
    DOIs
    Publication statusPublished - 2003

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