Maximized PUFA measurements improve insight in changes in fatty acid composition in response to temperature.

J.A. van Dooremalen, R. Pel, J. Ellers

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    A general mechanism underlying the response of ectotherms to environmental changes often involves changes in fatty acid composition. Theory predicts that a decrease in temperature causes an increase in unsaturation of fatty acids, with an important role for long-chain poly-unsaturated fatty acids (PUFAs). However, PUFAs are particularly unstable and susceptible to peroxidation, hence subtle differences in fatty acid composition can be challenging to detect. We determined the fatty acid composition in springtail (Collembola) in response to two temperatures (5°C and 25°C). First, we tested different sample preparation methods to maximize PUFAs. Treatments consisted of different solvents for primary lipid extraction, mixing with antioxidant, flushing with inert gas, and using different temperature exposures during saponification. Especially slow saponification at low temperature (90 min at 70°C) in combination with replacement of headspace air with nitrogen during saponification and methylation maximized PUFAs for GC analysis. Applying these methods to measure thermal responses in fatty acid composition, the data showed that the (maximized) proportion of C
    Original languageEnglish
    Pages (from-to)88-104
    JournalArchives of Insect Biochemistry and Physiology
    Volume72
    DOIs
    Publication statusPublished - 2009

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