TY - JOUR
T1 - On-line SPE-RP-LC for the determination of insulin derivatives in biological matrices
AU - Visser, N.F.C.
AU - Lingeman, H.
AU - Irth, H.
PY - 2003
Y1 - 2003
N2 - An automated and on-line solid-phase extraction (SPE)-liquid chromatography (LC) procedure is described for the determination of insulin in biological matrices. The total procedure consists of two SPEs in series, followed by RP-LC separation. During the first SPE a strong anion-exchange (SAX) cartridge (ISOLUTE, 40-90 μm, 10×4 mm i.d.) is used, followed by a RP-cartridge (Luna C
AB - An automated and on-line solid-phase extraction (SPE)-liquid chromatography (LC) procedure is described for the determination of insulin in biological matrices. The total procedure consists of two SPEs in series, followed by RP-LC separation. During the first SPE a strong anion-exchange (SAX) cartridge (ISOLUTE, 40-90 μm, 10×4 mm i.d.) is used, followed by a RP-cartridge (Luna C
U2 - 10.1016/S0731-7085(02)00730-6
DO - 10.1016/S0731-7085(02)00730-6
M3 - Article
SN - 0731-7085
VL - 32
SP - 295
EP - 309
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 2
ER -