Determination of isoflavone glucoside malonates in Trifolium pratense L. (red clover) extracts: quantification and stability studies

E. Drs. de Rijke, A. Zafra-Gomez, F. Ariese, U.A.T. Brinkman, C. Gooije

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Isoflavones, their glucosides and their glucoside malonates were determined in red clover leaf extracts using reversed-phase LC coupled to atmospheric pressure chemical ionisation mass spectrometry (APCI-MS), UV and fluorescence detectors and the stability of the malonates was investigated. Extracts can be stored at least 1-2 weeks at -20°C without loss of malonates. In LC-separated fractions the malonates are most stable when stored at low temperature after evaporation to dryness. The concentrations of eight major isoflavones ranged from 0.04 to 5 mg/g leaves. © 2001 Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)55-64
JournalJournal of Chromatography A
Volume932
Issue number1-2
DOIs
Publication statusPublished - 2001

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