TY - JOUR
T1 - Intermittent administration of morphine alters protein expression in rat nucleus accumbens
AU - Li, K.W.
AU - Jimenez, C.R.
AU - van der Schors, R.C.
AU - Hornshaw, M.P.
AU - Schoffelmeer, A.N.M.
AU - Smit, A.B.
PY - 2006
Y1 - 2006
N2 - Repeated exposure to drugs of abuse causes time-dependent neuroadaptive changes in the mesocorticolimbic system of the brain that are considered to underlie the expression of major behavioral characteristics of drug addiction. We used a 2-D gel-based proteomics approach to examine morphine-induced temporal changes in protein expression and/or PTM in the nucleus accumbens (NAc) of morphine-sensitized rats. Rats were pretreated with saline [1 mL/kg subcutaneously (s.c.)] or morphine (10mg/kg, s.c.) once daily for 14 days and the animals were decapitated 1 day later. The NAc was extracted and proteins resolved by 2-DE. Several protein functional groups were found to be regulated in the morphine-treated group, representing cytoskeletal proteins, proteins involved in neurotransmission, enzymes involved in energy metabolism and protein degradation, and a protein that regulates translation. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
AB - Repeated exposure to drugs of abuse causes time-dependent neuroadaptive changes in the mesocorticolimbic system of the brain that are considered to underlie the expression of major behavioral characteristics of drug addiction. We used a 2-D gel-based proteomics approach to examine morphine-induced temporal changes in protein expression and/or PTM in the nucleus accumbens (NAc) of morphine-sensitized rats. Rats were pretreated with saline [1 mL/kg subcutaneously (s.c.)] or morphine (10mg/kg, s.c.) once daily for 14 days and the animals were decapitated 1 day later. The NAc was extracted and proteins resolved by 2-DE. Several protein functional groups were found to be regulated in the morphine-treated group, representing cytoskeletal proteins, proteins involved in neurotransmission, enzymes involved in energy metabolism and protein degradation, and a protein that regulates translation. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
U2 - 10.1002/pmic.200500045
DO - 10.1002/pmic.200500045
M3 - Article
SN - 1874-3919
VL - 6
SP - 2003
EP - 2008
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -