Intermittent administration of morphine alters protein expression in rat nucleus accumbens

K.W. Li, C.R. Jimenez, R.C. van der Schors, M.P. Hornshaw, A.N.M. Schoffelmeer, A.B. Smit

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Repeated exposure to drugs of abuse causes time-dependent neuroadaptive changes in the mesocorticolimbic system of the brain that are considered to underlie the expression of major behavioral characteristics of drug addiction. We used a 2-D gel-based proteomics approach to examine morphine-induced temporal changes in protein expression and/or PTM in the nucleus accumbens (NAc) of morphine-sensitized rats. Rats were pretreated with saline [1 mL/kg subcutaneously (s.c.)] or morphine (10mg/kg, s.c.) once daily for 14 days and the animals were decapitated 1 day later. The NAc was extracted and proteins resolved by 2-DE. Several protein functional groups were found to be regulated in the morphine-treated group, representing cytoskeletal proteins, proteins involved in neurotransmission, enzymes involved in energy metabolism and protein degradation, and a protein that regulates translation. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
Original languageEnglish
Pages (from-to)2003-2008
Number of pages6
JournalJournal of Proteomics
Volume6
DOIs
Publication statusPublished - 2006

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