Multiple types of association of photosystem II and its light-harvesting antenna in partially solubilized photosystem II membranes.

E.J. Boekema, H. van Roon, F. Calkoen, R. Bassi, J.P. Dekker

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Photosystem II is a multisubunit pigment-protein complex embedded in the thylakoid membranes of chloroplasts. It utilizes light for photochemical energy conversion, and is heavily involved in the regulation of the energy flow. We investigated the structural organization of photosystem II and its associated light-harvesting antenna by electron microscopy, multivariate statistical analysis, and classification procedures on partially solubilized photosystem II membranes from spinach. Observation by electron microscopy shortly after a mild disruption of freshly prepared membranes with the detergent n-dodecyl-α,D-maltoside revealed the presence of several large supramolecular complexes. In addition to the previously reported supercomplexes [Boekema, E. J., van Roon, H., and Dekker, J. P. (1998) FEBS Lett. 424, 95-99], we observed complexes with the major trimeric chlorophyll a/b protein (LHCII) in a third, L-type of binding position (C
Original languageEnglish
Pages (from-to)2233-2239
JournalBiochemistry
Volume38
DOIs
Publication statusPublished - 1999

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