Evanescent-wave cavity ring-down detection of cytochrome c on surface-modified prisms
Sneppen, L. van der
MetadataShow full item record
Adsorption kinetics and molecular interactions on different Surface interfaces are studied by means of evanescent-wave cavity ring-down spectroscopy, using total internal reflection surfaces Onto Which different self-assembled monolayers are covalently attached. The adsorption of cytochrome c (a positively charged, spherical heme protein) to a negatively charged bare silica surface, as well as to C-18-coated (hydrophobic) and C3NH2-coated (positively charged) silica have been Studied. It is experimentally verified that these Surface layers do not interfere with the sensitive measurement of adsorbed cyt c monolayers Using the evanescent wave in a ring-down scheme. Attaching monolayers covalently to the silica total internal reflection Surface is a first step towards the development of a biosensor that makes use of immobilized biomolecules for specific detection of analytes in Solution. (C) 2009 Elsevier B.V. All rights reserved.