Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

M.T.A. Alexandre, T. Domratcheva, C. Bonetti, L. van Wilderen, M.L. Groot, R. van Grondelle, K.J. Hellingwerf, J.T.M. Kennis

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. Here, the primary reactions of Avena sativa phototropin 1 LOV2 (AsLOV2) were studied using ultrafast mid-infrared spectroscopy and quantum chemistry. The singlet excited state (S1) evolves into the triplet state (T1) with a lifetime of 1.5 ns at a yield of ̃50%. The infrared signature of S1 is characterized by absorption bands at 1657 cm
Original languageEnglish
Pages (from-to)227-237
JournalBiophysical Journal
Volume97
Issue number1
DOIs
Publication statusPublished - 2009

Bibliographical note

Primary Reactions of the LOV2 Domain of Phototropin Studied with Ultrafast Mid-infrared Spectroscopy and Quantum Chemistry

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